AMATA 2008: BioConductor / Limma Workshop
Location: The University of Otago Date: Saturday 15th November, 2008 Time: 9am - 3pm Course notes and light refreshments will be available to registrants. Places are limited, so please book early to avoid disappointment.
This tutorial will teach attendees to undertake basic differential expression analyses of microarray expression data using R and Bioconductor software. Normalization and quality assessment of Affymetrix, Illumina and two-colour microarray data will be covered. The emphasis will be on designed microarray experiments with a modest number of biological replicates, using the empirical Bayes approach of the limma package. After considering the simplest experimental designs, we will go on to consider multifactor experiments including time course experiments with multiple genotypes. Gene set testing and gene ontology analysis of differentially expressed genes will also be covered. The workshop is intended for bioinformaticians and for biologists unafraid of scripting languages. It will include an introduction to statistical programming using R, but attendees will benefit from some familiarity with R beforehand. The workshop will be conducted in the form of a hands-on computer lab session using a number of example microarray data sets.
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The Workshop will be conducted by Gordon Smyth and Mik Black.
